Improved molecular diagnostic tools for human hookworms

  title={Improved molecular diagnostic tools for human hookworms},
  author={Robin B. Gasser and Cinzia Cantacessi and Bronwyn Evelyn Campbell},
  journal={Expert Review of Molecular Diagnostics},
  pages={17 - 21}
Blood-feeding hookworms are parasitic roundworms (i.e., nematodes) of major socioeconomic importance, affecting millions of people worldwide. Despite their impact on human health, little attention has been paid to improving practical methods of diagnosis. The genetic characterization of hookworms and specific diagnosis of their infections are central to elucidating the ecology and epidemiology of these parasites as well as the control of the disease they cause. Traditional coprodiagnostic… 
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Differentiation between the human hookworms Ancylostoma duodenale and Necator americanus using PCR-RFLP.
  • J. Hawdon
  • Biology
    The Journal of parasitology
  • 1996
A PCR-based technique for the differentiation of hookworm species that infect humans is reported that is fast, simple, and hookworm specific and represents a considerable savings in time over current methods used for distinguishing between human hookworm infections.
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A review provides an account of some recent insights into the epidemiology and genetics of the parasite from human and non-human primate hosts in specific regions of Africa using molecular tools.
Specific amplification ofNecator americanusorAncylostoma duodenaleDNA by PCR using markers in ITS-1 rDNA, and its implications
The findings indicate clearly the potential for specific PCR to confirm the identity of eggs from faeces and larvae from the environment or host tissues and should have important implications for studying fundamental aspects relating to anthelmintic efficacy and the epidemiology of hookworms.