Improved methods to detect GTP-binding proteins from plants


Improved methods are described for the detection of G1P-binding proteins (G-proteins) in the protonema of mossFunaria hygrometrica and coleoptiles of corn(Zea mays) and sorghum(Sorghum vulgare). We optimized conditions for the transfer of proteins to nitrocellulose, production of high titer polyclonal anti-Gα (common) antibodies and finally the detection of… (More)
DOI: 10.1007/BF02720020


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@article{Panigrahi2007ImprovedMT, title={Improved methods to detect GTP-binding proteins from plants}, author={Kishore C. S. Panigrahi and Man Mohan Johri}, journal={Journal of Biosciences}, year={2007}, volume={23}, pages={193-200} }