Improved methods of cultivation and production of deuteriated proteins from E. coli strains grown on fully deuteriated minimal medium.

@article{Paliy2003ImprovedMO,
  title={Improved methods of cultivation and production of deuteriated proteins from E. coli strains grown on fully deuteriated minimal medium.},
  author={O Paliy and Deanna Bloor and David J Brockwell and Peter Gilbert and Jill Barber},
  journal={Journal of applied microbiology},
  year={2003},
  volume={94 4},
  pages={
          580-6
        }
}
AIMS The aim was to develop reliable and economical protocols for the production of fully deuteriated biomolecules by bacteria. This required the preparation of deuterium-tolerant bacterial strains and an understanding of the physiological mechanisms of acquisition of deuterium tolerance. METHODS AND RESULTS We report here improved methods for the cultivation of Escherichia coli on fully deuteriated minimal medium. A multi-stage adaptation protocol was developed; this included repeated… CONTINUE READING
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