Improved methodology for the detection and quantification of the acrosome reaction in mouse spermatozoa.

@article{Lybaert2009ImprovedMF,
  title={Improved methodology for the detection and quantification of the acrosome reaction in mouse spermatozoa.},
  author={P. Lybaert and A. Danguy and Fabienne Leleux and S. Meuris and P. Lebrun},
  journal={Histology and histopathology},
  year={2009},
  volume={24 8},
  pages={
          999-1007
        }
}
This study evaluates the use of two fluorescein-labelled (FITC) plant lectins, Pisum sativum (edible pea) agglutinin (PSA) and Arachis hypogaea (peanut) agglutinin (PNA), in order to determine the most accurate and reliable method to experimentally detect and assess the acrosome reaction in mouse spermatozoa. PNA-FITC labelling was restricted to the acrosome and was not influenced by the fixation procedure; either absolute methanol or paraformaldehyde. In contrast, PSA-FITC not only labelled… Expand
Methodological improvement of fluorescein isothiocyanate peanut agglutinin (FITC-PNA) acrosomal integrity staining for frozen-thawed Japanese Black bull spermatozoa
TLDR
Improved staining protocol of frozen-thawed Japanese Black bull sperm acrosomes with fluorescein isothiocyanate-conjugated peanut agglutinin will be useful to determine bull sperm Acrosomal integrity. Expand
Methodological factors affecting the results of staining frozen-thawed fertile and subfertile Japanese Black bull spermatozoa for acrosomal status.
TLDR
Important methodological considerations which need to be taken into account in order to design a reliable and reproducible protocol for the study of the acrosome are demonstrated. Expand
Characterization and potential roles of calretinin in rodent spermatozoa.
TLDR
The expression of calretinin in sperm is described for the first time and the potential implication of calcium-binding proteins in the sperm calcium-signaling cascade is extended and brought new insights into the understanding of sperm physiology. Expand
Peanut agglutinin specifically binds to a sperm region between the nucleus and mitochondria in tunicates and sea urchins
TLDR
It is concluded that ascidian sperm possess a non‐acrosomal, Triton‐resistant, glycan‐rich intracellular structure that may play a general role in reproduction of tunicates and sea urchins given its presence across a wide taxonomic range. Expand
Simultaneous Study of Cholesterol and GM1 Ganglioside by Specific Probes:Lipid Distribution during Maturation, Capacitation and the Acrosome Reaction
TLDR
Findings indicate that cholesterol and GM1 ganglioside have a dynamic behaviour during the life of the sperm, which could indicate that a continuous assembly or disassembly of the lipid raft could be involved in the responsiveness ofThe sperm to different environments. Expand
Changes in the Cellular Distribution of Tyrosine Phosphorylation and Its Relationship with the Acrosomal Exocytosis and Plasma Membrane Integrity during In Vitro Capacitation of Frozen/Thawed Bull Spermatozoa
TLDR
The results suggest that spontaneous AE and induced AE trigger similar cellular events regarding PTP and the spermatozoa showing PTP-EQ are more prone to suffer plasma membrane damage. Expand
Effectiveness of human spermatozoa biomarkers as indicators of structural damage during cryopreservation.
TLDR
It is found that the vitality, progressive motility and sperm count from low-quality samples after cryopreservation show higher damage rates than in normal sperm samples, and cytoskeleton, DNA, tail and mid-piece and acrosome display the highest cryodamage rates and are equally susceptible to cryop Reservation-induced damage in both low- and normal-quality semen samples. Expand
The Presence of D-Penicillamine during the In Vitro Capacitation of Stallion Spermatozoa Prolongs Hyperactive-Like Motility and Allows for Sperm Selection by Thermotaxis
TLDR
HTF induced higher levels of hyperactive-like motility and protein tyrosine phosphorylation (PTP) compared to the use of a medium commonly used in this species (Whitten’s), which is promising for improving the in vitro embryo production rates in these species by improving the sperm selection methodology. Expand
Proteomics of ionomycin‐induced ascidian sperm reaction: Released and exposed sperm proteins in the ascidian Ciona intestinalis
TLDR
In the exudate from sperm treated with ionomycin, membrane proteins including a possible VC receptor CiUrabin were abundant, indicating the release of membranous compartments during sperm reaction, and proteins containing a notable set of domains, astacin‐like metalloprotease domain and thrombospondin type 1 repeat(s), were found in this fraction. Expand
Successful delivery following intracytoplasmic sperm injection with calcium ionophore A23187 oocyte activation in a partially globozoospermic patient
TLDR
A successful pregnancy outcome is described following intracytoplasmic sperm injection (ICSI) with assisted oocyte activation (AOA) in a case of partial globozoospermia, which developed following ICSI using sperm from a partially Globozoospermic patient who possessed temporary potential oocytes activation. Expand
...
1
2
3
4
...

References

SHOWING 1-10 OF 40 REFERENCES
Assessment of the vitality and acrosomal status of human spermatozoa using fluorescent probes.
TLDR
Sperm vitality was assessed using H258 under different staining conditions and the HOS test and it was concluded that H258, as used in the H258/Con A-labelling method, was as good for vitality assessment as the E-N method employing eosin-staining for 15 sec. Expand
Specific labelling by peanut agglutinin of the outer acrosomal membrane of the human spermatozoon.
TLDR
It is concluded that PNA binds specifically to the outer acrosomal membrane, and that FITC-PNA-labelling may be used to monitor the human sperm acrosome reaction. Expand
Evaluation of mouse sperm acrosomal status and viability by flow cytometry
TLDR
The method developed provides an objective and efficient procedure to estimate simultaneously both acrosomal status and viability of mouse spermatozoa and was highly correlated with epifluorescence microscopic observation. Expand
Detection of human sperm acrosome reaction: comparison between methods using double staining, Pisum sativum agglutinin, concanavalin A and transmission electron microscopy.
TLDR
The percentages of spermatozoa with acrosomal loss detected by fluorescein isothiocyanate (FITC)-ConA were consistently lower than those obtained by double staining or FITC-PSA, which showed comparable results. Expand
Distinction between true acrosome reaction and degenerative acrosome loss by a one-step staining method using Pisum sativum agglutinin.
TLDR
The preferential whole-cell labelling of dead spermatozoa with PSA is considered to be due to increased availability of the nonacrosomal set of PSA-reactive sites in dead spermutozoa after a short treatment with methanol, whereas this treatment is probably not sufficient to expose most of these sites when applied to living spermatozosa. Expand
Combined use of fluorescent peanut agglutinin lectin and Hoechst 33258 to monitor the acrosomal status and vitality of human spermatozoa.
TLDR
The use of the fluorescent dye Hoechst 33258 (H33258) as a vital stain for use in combination with PNA-labelling was evaluated and it was found to provide vitality assessments comparable to those obtained using the standard eosin-exclusion method. Expand
Use of peanut agglutinin to assess the acrosomal status and the zona pellucida-induced acrosome reaction in stallion spermatozoa.
TLDR
A rapid and reliable assessment of the sperm acrosomal status and the incidence of the acrosome reaction of stallion spermatozoa at the zona surface were demonstrated in this study. Expand
Assessment of the acrosomal status of ram spermatozoa by RCA lectin-binding and partition in an aqueous two-phase system.
TLDR
The FITC-RCA-labeling procedure can be used to accurately assess the acrosomal status of ram spermatozoa in suspension and signals the presence of some deep membrane or intracellular residues that would affect partitioning. Expand
Two simple methods for detecting acrosome‐reacted human sperm
TLDR
Two methods for detecting acrosome reactions of human sperm at the light microscopic level are described, rapid, give similar results, and detect an increase in acrosomes reactions following exposure to the ionophore A23187. Expand
A new procedure for rapidly scoring acrosome reactions of human sperm
TLDR
It is concluded that the FITC-TCA labeling technique is a reliable method for accurately scoring the percentage of acrosome-reacted human sperm. Expand
...
1
2
3
4
...