Improved method for i.r. determination of the degree of N-acetylation of chitosan.

@article{Baxter1992ImprovedMF,
  title={Improved method for i.r. determination of the degree of N-acetylation of chitosan.},
  author={Andy J Baxter and M E Dillon and Kent D. Taylor and Gregory A Roberts},
  journal={International journal of biological macromolecules},
  year={1992},
  volume={14 3},
  pages={166-9}
}
Three published i.r. absorption band ratios for determining the % N-acetylation of chitosan have been used to follow the rate of alkaline deacetylation of chitin. Only one, the A1655/A3450 ratio, gives the expected rate of deacetylation curve. Consideration of the various i.r. ratios has led to a new relationship, % N-acetylation = (A1655/A3450) x 115, where the absorbance of the amide I band at approximately 1655 cm-1 is determined using the baseline proposed previously. The values obtained… CONTINUE READING