Improved method for evaluating the dead volume and protein-protein interactions by self-interaction chromatography.

@article{Binabaji2013ImprovedMF,
  title={Improved method for evaluating the dead volume and protein-protein interactions by self-interaction chromatography.},
  author={Elaheh Binabaji and Suma Rao and Andrew L Zydney},
  journal={Analytical chemistry},
  year={2013},
  volume={85 19},
  pages={9101-6}
}
Self-interaction chromatography (SIC) is a well-established method for studying protein-protein interactions. The second virial coefficient in SIC is evaluated directly from the measured retention coefficient for the protein using a column packed with resin on which the same protein has been immobilized on the pore surface. One of the challenges in determining the retention coefficient is the evaluation of the dead volume, which is the retention volume that would be measured for a… CONTINUE READING

References

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Fluid Phase Equilib

H. W. Blanch, J. M. Prausnitz, R. A. Curtis, D. Bratko
-1

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