Improved method for amino acid analysis of stained collagen bands from polyacrylamide gels using high-performance liquid chromatography.

Abstract

A procedure is described for the determination of the amino acid composition of stained collagen bands separated by sodium dodecyl sulphate polyacrylamide gel electrophoresis using high-performance liquid chromatography and post-labelling with o-phthalaldehyde. Six alpha-chain bands from collagens I, III and V were analysed directly after gel… (More)

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