Improved measurements of intracellular nitric oxide in intact microvessels using 4,5-diaminofluorescein diacetate.

@article{Zhou2011ImprovedMO,
  title={Improved measurements of intracellular nitric oxide in intact microvessels using 4,5-diaminofluorescein diacetate.},
  author={Xueping Zhou and Pingnian He},
  journal={American journal of physiology. Heart and circulatory physiology},
  year={2011},
  volume={301 1},
  pages={H108-14}
}
4,5-Diaminofluorescein diacetate (DAF-2 DA) has been widely used for the measurement of nitric oxide (NO) in living cells and tissues. We previously established a method that demonstrated platelet activating factor (PAF)-induced endothelial NO production in intact venules using DAF-2 DA. In previous applications, the loading dye was removed from the extracellular space before NO measurements. However, in high permeability vessels, endothelial cells quickly released the accumulated intracellular… CONTINUE READING
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