Improved mammalian expression systems by manipulating transcriptional termination regions.

@article{Kim2003ImprovedME,
  title={Improved mammalian expression systems by manipulating transcriptional termination regions.},
  author={Dongjun Kim and Jeong Do Kim and Kwanghee Baek and Yeup Yoon and Jaeseung Yoon},
  journal={Biotechnology progress},
  year={2003},
  volume={19 5},
  pages={1620-2}
}
Here we show that the transcriptional terminator element of human gastrin gene, which is the only element characterized to date in terms of its function in transcriptional termination, increases the transient expression levels of recombinant proteins. The expression of the beta-galactosidase gene was enhanced 3-4-fold in HeLa cells by inserting the terminator element of human gastrin gene at the 3'-side of the SV40 polyadenylation signal/cleavage site of the control vector (pSV-beta-gal). This… CONTINUE READING

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