Improved liquid-chromatographic determination of caffeine in plasma.

@article{Blanchard1980ImprovedLD,
  title={Improved liquid-chromatographic determination of caffeine in plasma.},
  author={Jocelyn Blanchard and J D Mohammadi and Kenneth A. Conrad},
  journal={Clinical chemistry},
  year={1980},
  volume={26 9},
  pages={1351-4}
}
We describe a rapid, specific, and sensitive liquid-chromatographic micro-method for caffeine in plasma. Each plasma sample can be assayed within about 15 min of its receipt. Samples are denatured with acetonitrile, centrifuged, and the supernate is chromatographed on a reversed-phase column. Only 100 microL of plasma is required, and concentrations as low as 0.3 mg/L can be measured accurately. Other xanthines and their metabolites do not interfere. The small sample required makes the… CONTINUE READING

From This Paper

Topics from this paper.

Citations

Publications citing this paper.
Showing 1-9 of 9 extracted citations

The absolute bioavailability of caffeine in man

European Journal of Clinical Pharmacology • 1983
View 1 Excerpt

Similar Papers

Loading similar papers…