Improved leader and putative terminator sequences for high-level production of Streptomyces subtilisin inhibitor in Escherichia coli

Abstract

A high-level production system in Escherichia coli for an alkaline serine protease inhibitor, termed Streptomyces subtilisin inhibitor (SSI), from S. albogriseolus S-3253 was established by replacing the SSI signal sequence with the OmpA signal sequence using the inducible pIN-III-ompA vector. Significant amounts of recombinant SSI, resulting from accurate… (More)
DOI: 10.1007/BF00164458

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