Improved inverse PCR scheme for metagenome walking.

@article{Uchiyama2006ImprovedIP,
  title={Improved inverse PCR scheme for metagenome walking.},
  author={Taku Uchiyama and Kazuya Watanabe},
  journal={BioTechniques},
  year={2006},
  volume={41 2},
  pages={
          183-8
        }
}
Inverse PCR has been used for the recovery of genome regions flanking a known sequence, although its application to metagenome walking is limited due to inefficient amplification from low copy number fragments. Here we present an improved inverse PCR scheme that enables walking of rare fragments in environmental metagenomes. Our scheme includes the following steps: (i) inverse PCR in which one primer is connected to an affinity tag; (ii) affinity purification of PCR products for removing… CONTINUE READING

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