Improved indirect fluorescence immunocytochemical method using counterstains.

@article{Newkirk1992ImprovedIF,
  title={Improved indirect fluorescence immunocytochemical method using counterstains.},
  author={Robert F. Newkirk and James Mack},
  journal={BioTechniques},
  year={1992},
  volume={13 4},
  pages={536-8}
}
Immunocytochemistry in recent years has provided powerful tools for research in neurobiology. One of the more popular techniques is the indirect fluorescence technique in which fluorescein isothiocyanate (FITC) or tetrahodamine isothiocyanate (TRITC) is used. Although widely used, this technique has two disadvantages: (1) localization may be difficult in relation to background morphology, and (2) the fluorescence fades. The study reported here describes a modification of an indirect… CONTINUE READING

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