Improved in vitro models for assay of rheumatoid synoviocyte chemotaxis.

@article{Wang1994ImprovedIV,
  title={Improved in vitro models for assay of rheumatoid synoviocyte chemotaxis.},
  author={A. Z. Wang and J M Chen and Gregory W. Fisher and John Yuan Wang and Herbert S. Diamond},
  journal={Clinical and experimental rheumatology},
  year={1994},
  volume={12 3},
  pages={293-9}
}
OBJECTIVES (1) To define the optimal conditions for transwell assay of synoviocyte chemotaxis. (2) To develop a live cell imaging chemotaxis assay for synoviocytes. (3) To characterize the chemotaxis of rheumatoid synoviocytes (RAS). RESULTS Optimal conditions for transwell assay of synoviocyte chemotaxis were 8 microns pore size filters coated with collagen I (C1), assayed for 24 hours. Without the C1 coating 2.9 x 10(3) RAS migrated to the lower chamber. This increased to 4.7 x 10(3) when… CONTINUE READING