Improved in vitro development of porcine embryos with different energy substrates and serum.

@article{Kim2004ImprovedIV,
  title={Improved in vitro development of porcine embryos with different energy substrates and serum.},
  author={Hye-Soo Kim and Gab-sang Lee and Sang-hwan Hyun and So-hyun Lee and Dong-Hyun Nam and Yeon-woo Jeong and Sue Kim and Sung-Keun Kang and Byeong Chun Lee and Woo-suk Hwang},
  journal={Theriogenology},
  year={2004},
  volume={61 7-8},
  pages={1381-93}
}
The effect of replacing 5.5 mM glucose in North Carolina State University (NCSU)-23 medium with 0.5 mM pyruvate/5.0 mM lactate on porcine IVF embryo development was investigated in Experiment 1. Culturing embryos with pyruvate/lactate for 7 days or with pyruvate/lactate from Days 0 to 2, and then glucose from Days 2 to 7 improved cleavage rates. In Experiment 2, embryos were cultured for 7 days in pyruvate/lactate containing NCSU-23 medium supplemented with 0.05% PVA, 0.4% BSA or 10% fetal… CONTINUE READING