Improved identification of antitrypsin phenotypes through isoelectric focusing with dithioerythritol.

  title={Improved identification of antitrypsin phenotypes through isoelectric focusing with dithioerythritol.},
  author={John A. Pierce and B G Eradio},
  journal={The Journal of laboratory and clinical medicine},
  volume={94 6},
Isoelectric focusing has replaced acid starch-gel electrophoresis for the routine determination of antitrypsin phenotypes in recent years. We observed increased sharpness of antitrypsin bands and decreased background stain following the addition of DTE to serum before isoelectric ofcusing in polyacrylamide gels. DTE and DTT were the only sulfhydryl reducing agents which produced this effect. Electrophoretic mobility of antitrypsin bands was increased very slightly. Decreased background stain… CONTINUE READING


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