Improved high-force magnetic tweezers for stretching and refolding of proteins and short DNA.

@article{Chen2011ImprovedHM,
  title={Improved high-force magnetic tweezers for stretching and refolding of proteins and short DNA.},
  author={Hu Chen and Hongxia Fu and Xiaoying Zhu and Peiwen Cong and Fumihiko Nakamura and Jie S. Yan},
  journal={Biophysical journal},
  year={2011},
  volume={100 2},
  pages={517-23}
}
Although magnetic tweezers have many unique advantages in terms of specificity, throughput, and force stability, this tool has had limited application on short tethers because accurate measurement of force has been difficult for short tethers under large tension. Here, we report a method that allows us to apply magnetic tweezers to stretch short biomolecules with accurate force calibration over a wide range of up to 100 pN. We demonstrate the use of the method by overstretching of a short DNA… CONTINUE READING

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