Improved heterologous protein expression in the chloroplast of Chlamydomonas reinhardtii through promoter and 5' untranslated region optimization.

@article{Rasala2011ImprovedHP,
  title={Improved heterologous protein expression in the chloroplast of Chlamydomonas reinhardtii through promoter and 5' untranslated region optimization.},
  author={Beth A. Rasala and Machiko Muto and Joseph Sullivan and Stephen Patrick Mayfield},
  journal={Plant biotechnology journal},
  year={2011},
  volume={9 6},
  pages={674-83}
}
Microalgae have the potential to be a valuable biotechnological platform for the production of recombinant proteins. However, because of the complex regulatory network that tightly controls chloroplast gene expression, heterologous protein accumulation in a wild-type, photosynthetic-competent algal chloroplast remains low. High levels of heterologous protein accumulation have been achieved using the psbA promoter/5' untranslated region (UTR), but only in a psbA-deficient genetic background… CONTINUE READING

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