Improved hatching for in vitro quail embryo culture using surrogate eggshell and artificial vessel.

@article{Kamihira1998ImprovedHF,
  title={Improved hatching for in vitro quail embryo culture using surrogate eggshell and artificial vessel.},
  author={Masamichi Kamihira and Satoshi Oguchi and Akira Tachibana and Yuichiro Kitagawa and Shinji Iijima},
  journal={Development, growth & differentiation},
  year={1998},
  volume={40 4},
  pages={449-55}
}
The establishment of avian embryonic culture is important both for the analysis of the developmental process and the establishment of transgenic chickens that produce useful biological materials in eggs. However, the hatchability of cultured embryos has been approximately 50%. We identified that the low rate of hatchability of cultured embryos was caused by limited oxygen and calcium availability. In quail embryo culture using chicken eggshell as a culture vessel, viability in the middle stage… CONTINUE READING

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