Improved genetic transformation protocol for cork oak (Quercus suber L.)


An optimized protocol for Agrobacterium tumefaciens-mediated transformation of mature Quercus suber L. embryogenic masses is reported. In this work several variables were tested. Plant genotype, explant type and time elapsed between the last subculture and inoculation, i.e. the explant preculture period, were found to be very important. Interaction between… (More)
DOI: 10.1007/s11240-007-9276-6

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