Improved genetic transformation of the thermophilic cyanobacterium, Thermosynechococcus elongatus BP-1.

Abstract

We improved genetic transformation of the thermophilic cyanobacterium, Thermosynechococcus elongatus BP-1, by combining electroporation with a top agar method. Transformation was also improved when a disruptant of a putative type I restriction endonuclease (tll2230) was used as recipient cells. In particular, some constructs, with which wild type has never been transformed, were successfully integrated into the tll2230-disruptant. Single-crossover recombination was detected more frequently than the double-crossover recombination. In accordance with the presence of all the homologs of pil genes in Synechocystis sp. PCC 6803, we found that T. elongatus is naturally transformable with exogenous DNA.

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@article{Iwai2004ImprovedGT, title={Improved genetic transformation of the thermophilic cyanobacterium, Thermosynechococcus elongatus BP-1.}, author={Masako Iwai and Hiroshi Katoh and Mitsunori Katayama and Masahiko Ikeuchi}, journal={Plant & cell physiology}, year={2004}, volume={45 2}, pages={171-5} }