Improved frost tolerance and winter survival in winter barley (Hordeum vulgare L.) by in vitro selection of proline overaccumulating lines

@article{Tantau2004ImprovedFT,
  title={Improved frost tolerance and winter survival in winter barley (Hordeum vulgare L.) by in vitro selection of proline overaccumulating lines},
  author={Hanny Tantau and Christiane C Balko and B{\"a}rbel Brettschneider and Gilbert Melz and Karl Doerffling},
  journal={Euphytica},
  year={2004},
  volume={139},
  pages={19-32}
}
Embryogenic calli derived from anther cultures of the two-rowed winter barley cultivar ‘Igri’ were plated on solid L3 medium containing the proline analogue hydroxyproline (Hyp), 10–20 mmol l−1. Exposure to Hyp caused severe degeneration of most of the calli. Hyp resistant calli, distinguishable by their lighter colour and higher growth rate, and control calli not exposed to Hyp were plated on L3 regeneration medium. From 22,500 anthers exposed to Hyp 46 Hyp resistant regenerates were obtained… CONTINUE READING
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