Improved fluorescence in situ hybridization of individual microbial cells using polynucleotide probes: the network hypothesis.

@article{Zwirglmaier2003ImprovedFI,
  title={Improved fluorescence in situ hybridization of individual microbial cells using polynucleotide probes: the network hypothesis.},
  author={Katrin Zwirglmaier and Wolfgang Ludwig and Karl Heinz Schleifer},
  journal={Systematic and applied microbiology},
  year={2003},
  volume={26 3},
  pages={327-37}
}
Fluorescence in situ hybridizations using polynucleotide transcript probes (poly-FISH) usually exhibit a ring-shaped halo or corona-like fluorescence signal, whereas hybridizations with oligonucleotide probes (oligo-FISH) result in a uniform and evenly distributed fluorescence throughout the cell. The superiority of poly-FISH in comparison to oligo-FISH regarding the signal intensity and the detection of cells with a low ribosome content suggested a further investigation of the possibilities of… CONTINUE READING

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