Improved fixation and cobalt-glucose oxidase-diaminobenzidine intensification for immunohistochemical demonstration of corticotropin-releasing factor in rat brain.

@article{Sakanaka1987ImprovedFA,
  title={Improved fixation and cobalt-glucose oxidase-diaminobenzidine intensification for immunohistochemical demonstration of corticotropin-releasing factor in rat brain.},
  author={Masahiro Sakanaka and Tamotsu Shibasaki and Karl P. Lederis},
  journal={The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society},
  year={1987},
  volume={35 2},
  pages={207-12}
}
An optimal fixation method and intensification procedure may be required in brain immunohistochemistry to obtain intense and widespread staining for a specific antigen, in cases where ordinary fixation and conventional immunohistochemistry result in only partial demonstration of the antigen. In the present study of localization of corticotropin-releasing factor immunoreactivity (CRFI) in rat brain, the importance of such intensification is shown. We describe a fixation procedure in which… CONTINUE READING

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