Improved expression and immobilization of Geobacillus thermoleovorans CCR11 thermostable recombinant lipase.

@article{BadilloZeferino2017ImprovedEA,
  title={Improved expression and immobilization of Geobacillus thermoleovorans CCR11 thermostable recombinant lipase.},
  author={Giselle-Lilian Badillo-Zeferino and Irving-Israel Ruiz-L{\'o}pez and Rosamaŕıa Oliart-Ros and Mar{\'i}a-Guadalupe S{\'a}nchez-Otero},
  journal={Biotechnology and applied biochemistry},
  year={2017},
  volume={64 1},
  pages={
          62-69
        }
}
Production of recombinant thermo-alkali-stable lipase LipMatCCR11, expressed in Escherichia coli BL21 (DE3), was investigated via response surface methodology by using a face-centered design with three levels of each factor. Additionally, improvement of the catalytic performance of expressed lipase was assessed by immobilization on microporous polypropylene. Results showed that inducer (isopropyl β-d-1-thiogalactopyranoside [IPTG]) concentration and temperature were found to be the significant… CONTINUE READING
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