Improved estimation of cholesteryl ester transfer/exchange activity in serum or plasma.

@article{Groener1986ImprovedEO,
  title={Improved estimation of cholesteryl ester transfer/exchange activity in serum or plasma.},
  author={Johanna E. M. Groener and Ron W. Pelton and Gert M. Kostner},
  journal={Clinical chemistry},
  year={1986},
  volume={32 2},
  pages={283-6}
}
This simple, routine assay for measuring cholesteryl ester transfer/exchange activity in human plasma is based on the removal of interfering lipoproteins--very-low-density (VLDL) and low-density lipoproteins (LDL)--by precipitation with polyethylene glycol. High-density lipoproteins (HDL) in the samples do not affect the results. The supernate after precipitation is mixed with [14C]cholesteryl ester-labeled LDL as donor and with HDL as the acceptor for the cholesteryl ester. After incubation… CONTINUE READING

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