Improved enzymatic isolation of fibroblasts for the creation of autologous skin substitutes

  title={Improved enzymatic isolation of fibroblasts for the creation of autologous skin substitutes},
  author={Hongjun Wang and Clemens A. van Blitterswijk and Marion Bertrand-De Haas and Arnold H. Schuurman and Evert N. Lamme},
  journal={In Vitro Cellular & Developmental Biology - Animal},
The number of medical applications using autologous fibroblasts is increasing rapidly. We investigated thoroughly the procedure to isolate cells from skin using the enzymatic tissue dissociation procedure. Tissue digestion efficiency, cell viability, and yield were investigated in relation to size of tissue fragments, digestion volume to tissue ratio, digestion time, and importance of other protease activities present in Clostridium histolyticum collagenase (CHC) (neutral protease, clostripain… CONTINUE READING


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