Improved electroporation and cloning vector system for gram-positive bacteria.

  title={Improved electroporation and cloning vector system for gram-positive bacteria.},
  author={Gary M Dunny and Linda N. Lee and Donald J Leblanc},
  journal={Applied and environmental microbiology},
  volume={57 4},
A protocol for transformation of intact Enterococcus faecalis cells by electroporation was developed through a systematic examination of the effects of changes in various parameters, including (i) growth conditions; (ii) composition of the electroporation solution; (iii) electroporation conditions, such as field strength and resistance; (iv) size, concentration, and purity of DNA used for transformation; and (v) conditions used to select for transformants. Key features of this protocol include… CONTINUE READING
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