Improved electrophoresis and transfer of picogram amounts of protein with hemoglobin.

@article{Gillespie1997ImprovedEA,
  title={Improved electrophoresis and transfer of picogram amounts of protein with hemoglobin.},
  author={Peter G. Gillespie and Susan K.H Gillespie},
  journal={Analytical biochemistry},
  year={1997},
  volume={246 2},
  pages={239-45}
}
During electrophoresis and electroblotting to transfer membranes, picogram amounts of protein can react irreversibly with the polyacrylamide matrix, preventing complete electrophoresis and efficient electroblotting. Bovine hemoglobin, but not other potential carrier proteins, mitigates this protein loss by migrating with or ahead of other proteins and… CONTINUE READING