Improved efficiency of doubled haploid generation in hexaploid triticale by in vitro chromosome doubling

@inproceedings{Wrschum2012ImprovedEO,
  title={Improved efficiency of doubled haploid generation in hexaploid triticale by in vitro chromosome doubling},
  author={Tobias W{\"u}rschum and Matthew R Tucker and Jochen Christoph Reif and Hans Peter Maurer},
  booktitle={BMC Plant Biology},
  year={2012}
}
BackgroundDoubled haploid production is a key technology in triticale research and breeding. A critical component of this method depends on chromosome doubling, which is traditionally achieved by in vivo treatment of seedlings with colchicine.ResultsIn this study we investigated the applicability of an in vitro approach for chromosome doubling based on microspore culture. Our results show a pronounced increase in the proportion of doubled haploid triticale plants compared to the spontaneous… CONTINUE READING

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