Improved drought tolerance of transgenic Zea mays plants that express the glutamate dehydrogenase gene (gdhA) of E. coli

  title={Improved drought tolerance of transgenic Zea mays plants that express the glutamate dehydrogenase gene (gdhA) of E. coli},
  author={David A. Lightfoot and Rajsree Mungur and Rafiqa Ameziane and Scott A. Nolte and Lynn M. Long and Karen Bernhard and and J S Colter and Karen Jones and Muhammad J. Iqbal and E. C. Varsa and Brian Young},
Genetic modification of nitrogen metabolism via bacterial NADPH- dependent glutamate dehydrogenase (GDH; E.C. favorably alters growth and metabolism of C3 plants. The aim of this study was to examine the effect of expression of GDH in the cytoplasmic compartment of Zea mays cells. The gdhA gene from Escherichia coli , that encoded a NADPH-GDH, was ligated to the ubiquitin promoter that incorporated the first intron enhancer and used to transform Z. mays cv. ‘H99’ embryo cultures by… 
Resolving the role of plant glutamate dehydrogenase: II. Physiological characterization of plants overexpressing the two enzyme subunits individually or simultaneously.
Overexpressing the two genes GDHA and GDHB individually or simultaneously induced a differential accumulation of glutamate and glutamine and a modification of the glutamate to glutamine ratio, which may explain the lower plant biomass determined in the GDH-overexpressing lines.
Resolving the Role of Plant NAD-Glutamate Dehydrogenase: III. Overexpressing Individually or Simultaneously the Two Enzyme Subunits Under Salt Stress Induces Changes in the Leaf Metabolic Profile and Increases Plant Biomass Production.
Metabolomic analysis revealed that overexpressing the two genes GDHA and GDHB, individually or simultaneously, induced a differential accumulation of several carbon- and nitrogen-containing molecules involved in a variety of metabolic, developmental and stress-responsive processes.
Introduction of a fungal NADP(H)-dependent glutamate dehydrogenase (gdhA) improves growth, grain weight and salt resistance by enhancing the nitrogen uptake efficiency in forage rice
The present study showed that the introduction of a fungal gdhA into forage rice could lead to higher source ability, better growth and higher grain weight by enhancing nitrogen uptake efficiency.
The effects of introduction of a fungal glutamate dehydrogenase gene (gdhA) on the photosynthetic rates, biomass, carbon and nitrogen contents in transgenic potato
It is shown that the gdhA gene is a powerful tool to increase tuber dry matter and improve eciency of nitrogen use of potato and there were higher NADP(H)-GDH activities in GDH potato leaves than in the wild type.
Over-expression of a glutamate dehydrogenase gene, MgGDH, from Magnaporthe grisea confers tolerance to dehydration stress in transgenic rice
Results indicate that heterologous expression of MgGDH can prevent toxic accumulation of ammonium and in return improve dehydration stress tolerance in rice.
A quantitative genetic study for elucidating the contribution of glutamine synthetase, glutamate dehydrogenase and other nitrogen-related physiological traits to the agronomic performance of common wheat
This work investigated the role of the enzymes glutamine synthetase and glutamate dehydrogenase, and other nitrogen-related physiological traits in the control of agronomic performance in wheat, and proposed possible functions of physiological traits to explain the variation observed for Agronomic traits including yield and its components.
Over-expression of a fungal NADP(H)-dependent glutamate dehydrogenase PcGDH improves nitrogen assimilation and growth quality in rice
It is demonstrated that heterologous expression of P. cystidiosus GDH (PcGDH) could improve nitrogen assimilation and growth in rice and also interconvert 2-OG and glutamate.
TRANSFORMATION OF Escherichia coli MODIFIED gdhA GENE INTO Nicotiana tabacum
The gdhA gene from Escherichia coli encoding a NADPH-GDH was expressed in tobacco plants exhibited high growth performance and enhanced herbicide resistance as well as drought tolerance and suggested that it can be transformed into other crops such as maize.
Abiotic Stresses Downregulate Key Genes Involved in Nitrogen Uptake and Assimilation in Brassica juncea L.
Observations indicate that expression of most of the genes is adversely affected under abiotic stress conditions, particularly under prolonged stress exposure (24h), which may be one of the reasons of reduction in plant growth and development under abiotics stresses.
Physiological and Molecular Osmotic Stress Responses in Three Durum Wheat (Triticum Turgidum ssp Durum) Genotypes
Interestingly, Maali and YT13 showed higher transcript abundance for GDH under stress compared to ON66, suggesting the implication of GDH in protective phenomena upon osmotic stress.


Expression of the bacterial gdhA gene encoding a NADPH glutamate dehydrogenase in tobacco affects plant growth and development
The modifications in transgenic plants may result from both increased nitrogen efficiency and altered gene expression and metabolism, suggesting that both nitrogen and carbon metabolism were altered.
The glutamate dehydrogenase gene gdhA increased the resistance of tobacco to glufosinate
Summary The gene gdhA from Escherichia coli, that encodes a NADPH-dependent glutamate dehydrogenase (GDH), directs a novel pathway in transgenic plants that allows an increase in ammonium
Highly effective expression of glutamine synthetase genes GS1 and GS2 in transgenic rice plants increases nitrogen-deficiency tolerance.
  • Hui Sun, Q. Huang, J. Su
  • Biology, Medicine
    Zhi wu sheng li yu fen zi sheng wu xue xue bao = Journal of plant physiology and molecular biology
  • 2005
The result suggests that expression of p2GS makes the transgenic rice plants tolerant to nitrogen-deficiency, and the transcription of GS1-GS2 genes in the transformants was confirmed by Northern blot analysis.
Water potential is maintained during water deficit in Nicotiana tabacum expressing the Escherichia coli glutamate dehydrogenase gene
Expression of bacterial gdhA (glutamate dehydrogenase; GDH; E.C. genes in transgenic plants fundamentally alters plant growth, herbicide tolerance and metabolite profiles. The aim was to
New insights towards the function of glutamate dehydrogenase revealed during source-sink transition of tobacco (Nicotiana tabacum) plants grown under different nitrogen regimes.
It is concluded that GDH does not play a direct role during the process of nitrogen remobilization but is rather induced following a built up of ammonium provided externally or released as the result of protein hydrolysis during natural leaf senescence.
Glutamate Dehydrogenase of Tobacco Is Mainly Induced in the Cytosol of Phloem Companion Cells When Ammonia Is Provided Either Externally or Released during Photorespiration
The results suggest that the enzyme plays a dual role in companion cells, either in the mitochondria when mineral nitrogen availability is low or in the cytosol when ammonium concentration increases above a certain threshold.
Glutamine synthetase and glutamate dehydrogenase isoforms in maize leaves: localization, relative proportion and their role in ammonium assimilation or nitrogen transport
A possible role for GS-1 and GDH co-acting in the synthesis of glutamine for the transport of nitrogen is discussed, with results consistent with the known intercellular distribution of nitrate reductase and Fd-GOGAT proteins in maize leaves.
Physiology of maize II: Identification of physiological markers representative of the nitrogen status of maize (Zea mays) leaves during grain filling
There is a strong correlation between total N, chlorophyll, soluble protein and GS activity, which is not dependent upon the N fertilization level, which indicates the N status of the plant, either in a single leaf or during ageing.
Overexpression of a soybean cytosolic glutamine synthetase gene linked to organ-specific promoters in pea plants grown in different concentrations of nitrate
It is indicated that overexpression of GS15 in various tissues of pea does not consistently result in increases in GS activity and that the increase in root GS activity is not always consistent with decreases in plant N and biomass accumulation and that further investigation of the relationship between rootGS activity and growth responses is warranted.
Does root glutamine synthetase control plant biomass production in Lotus japonicus L.?
It was confirmed that a negative relationship exists between root GS expression and plant biomass production in both the two parental lines and their progeny and it was estimated that at least 13% of plant growth variation can be accounted for by variation in GS activity.