Improved detection of rotavirus shedding by polymerase chain reaction.

  title={Improved detection of rotavirus shedding by polymerase chain reaction.},
  author={James O Wilde and Robert Yolken and R. Willoughby and Joseph E Eiden},
  volume={337 8737},
To improve identification of children excreting rotavirus a method for the amplification of rotavirus RNA by the polymerase chain reaction (PCR) was developed. The assay was compared with a solid-phase enzyme immunoassay in the detection of rotavirus shedding by infants in hospital during the winter peak of rotavirus infections. Forty children were studied in an intermediate care unit after transfer from intensive care units. Only two were admitted primarily because of diarrhoea; the other… CONTINUE READING
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