Improved detection of microbial DNA after bead-beating before DNA isolation.

@article{Boer2010ImprovedDO,
  title={Improved detection of microbial DNA after bead-beating before DNA isolation.},
  author={Richard F. de Boer and Remco P H Peters and Sonja Gierveld and Tim Schuurman and Mirjam A M D Kooistra-Smid and Paul H. M. Savelkoul},
  journal={Journal of microbiological methods},
  year={2010},
  volume={80 2},
  pages={209-11}
}
Detection of microbial DNA in clinical samples by real-time PCR requires a universal extraction method with equally efficient lysis of cell walls of all possible microorganisms. We demonstrated that physical disruption by bead-beating with 0.1mm beads in combination with MagNA Pure DNA III extraction enhances microbial lysis of diverse Gram-positive microorganisms and may be used to optimize DNA extraction protocols in routine clinical diagnostics.