Improved detection of amylase activity by sodium dodecyl sulfate-polyacrylamide gel electrophoresis with copolymerized starch.

@article{Martnez2000ImprovedDO,
  title={Improved detection of amylase activity by sodium dodecyl sulfate-polyacrylamide gel electrophoresis with copolymerized starch.},
  author={Tom{\'a}s Adri{\'a}n Alonso Mart{\'i}nez and F Javier Alarc{\'o}n and Manuel D{\'i}az-L{\'o}pez and Francisco Javier Moyano},
  journal={Electrophoresis},
  year={2000},
  volume={21 14},
  pages={
          2940-3
        }
}
An improved method, based on sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) for detection of amylase activity is described. This method will allow better characterization of certain amylases than that obtained by the Davis technique. The main features of the technique are: (i) identification of amylase bands and molecular mass determination are possible in the same gel; (ii) the hydrolysis of copolymerized substrate during electrophoretic separation is prevented using very… CONTINUE READING
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