Improved detection of Candida albicans by PCR in blood of neutropenic mice with systemic candidiasis.

@article{Deventer1995ImprovedDO,
  title={Improved detection of Candida albicans by PCR in blood of neutropenic mice with systemic candidiasis.},
  author={A J van Deventer and Wil H. F. Goessens and Alex van Belkum and Hans J van Vliet and Els W M van Etten and Henri A. Verbrugh},
  journal={Journal of clinical microbiology},
  year={1995},
  volume={33 3},
  pages={625-8}
}
A PCR using primers aimed at the multicopy gene coding for the small subunit rRNA and resulting in the synthesis of a 180-bp fragment was evaluated for its use in diagnosing invasive candidiasis in comparison with blood culture. With the use of a C. albicans-specific probe, +/- 10 to 15 C. albicans cells are detected in 100 microliters of whole blood by Southern analysis. A DNase pretreatment was critical in the purification process of yeast DNA from whole blood. Omission of the DNase… CONTINUE READING