Improved detection and quantification of mouse cytomegalovirus by real-time PCR.

@article{Vliegen2003ImprovedDA,
  title={Improved detection and quantification of mouse cytomegalovirus by real-time PCR.},
  author={Inge Vliegen and Selma B Herngreen and G. E. L. M. Grauls and C. A. M. V. A. Bruggeman and Frank R M Stassen},
  journal={Virus research},
  year={2003},
  volume={98 1},
  pages={17-25}
}
During latent cytomegalovirus (CMV) infection, viral presence cannot be detected by plaque assay. Therefore, we assessed the applicability of real-time PCR for temporal determination of virus dissemination in two different mouse strains. Eight-week-old BALB/c and C57BL/6J mice were infected with mouse CMV (MCMV) and sacrificed at 1, 2, 4, 6, 14 and 28 days post infection. Real-time PCR was used to determine MCMV copy number in the heart, bone marrow cells, aorta and blood. In lung, liver… CONTINUE READING