Improved cryopreservation of oil palm (Elaeis guineensis Jacq.) polyembryoids using droplet vitrification approach and assessment of genetic fidelity

@article{Gantait2014ImprovedCO,
  title={Improved cryopreservation of oil palm (Elaeis guineensis Jacq.) polyembryoids using droplet vitrification approach and assessment of genetic fidelity},
  author={Saikat Gantait and Uma Rani Sinniah and Periasamy Suranthran and Sharrmila Rengeswari Palanyandy and Sreeramanan Subramaniam},
  journal={Protoplasma},
  year={2014},
  volume={252},
  pages={89-101}
}
In the present study, polyembryoids of oil palm (Elaeis guineensis Jacq.) were cryopreserved with successful revival of 68 % for the first time using the droplet vitrification technique. Excised polyembryoids (3–5-mm diameter) from 3-month-old in vitro cultures were pre-cultured for 12 h in liquid Murashige and Skoog medium supplemented with 0.5 M sucrose. The polyembryoids were osmoprotected in loading solution [10 % (w/v) dimethyl sulphoxide (DMSO) plus 0.7 M sucrose] for 30 min at room… CONTINUE READING

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