Improved conditions for extraction and amplification of human immunodeficiency virus type 1 RNA from plasma samples with low viral load.

@article{Villahermosa2000ImprovedCF,
  title={Improved conditions for extraction and amplification of human immunodeficiency virus type 1 RNA from plasma samples with low viral load.},
  author={Mar{\'i}a Luisa Villahermosa and Michael M. Thomson and Elena V{\'a}zquez de Parga and Mar{\'i}a Teresa Cuevas and G Paniagua Contreras and Luc{\'i}a P{\'e}rez-{\'A}lvarez and Elena Delgado and Nuria Manj{\'o}n and Leandro Medrano and Rafael N{\'a}jera},
  journal={Journal of human virology},
  year={2000},
  volume={3 1},
  pages={27-34}
}
OBJECTIVES We attempted to define optimal conditions for amplification of low copy number HIV-1 RNA sequences in plasma samples, applying improved conditions for nucleic acid extraction and amplification. METHODS Several methodologic parameters were evaluated, including methods of RNA extraction, volumes of plasma samples, proportion of extracted RNA used as a template for amplification, and reverse transcriptase-DNA polymerase enzyme combination employed in cDNA synthesis and polymerase… CONTINUE READING

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