Improved co-expression of multiple genes in vectors containing internal ribosome entry sites (IRESes) from human genes

@article{Wong2002ImprovedCO,
  title={Improved co-expression of multiple genes in vectors containing internal ribosome entry sites (IRESes) from human genes},
  author={E Wong and S S Ngoi and CGL Lee},
  journal={Gene Therapy},
  year={2002},
  volume={9},
  pages={337-344}
}
Incorporation of an internal ribosome entry site (IRES) into the gene therapy vector represents a promising strategy to efficiently co-express several gene products from the same promoter. However, vector systems that utilize the encephalomyocarditis virus IRES express the downstream gene much less efficiently than the upstream gene. In this study, we compared four IRESes isolated from human genes against the EMCV IRES, using β-galactosidase and chloramphenicol acetyl transferase genes as… CONTINUE READING