Improved cloning and expression of cytochrome P450s and cytochrome P450 reductase in yeast.

@article{Hamann2007ImprovedCA,
  title={Improved cloning and expression of cytochrome P450s and cytochrome P450 reductase in yeast.},
  author={Thomas Hamann and Birger Lindberg M\oller},
  journal={Protein expression and purification},
  year={2007},
  volume={56 1},
  pages={121-7}
}
Combination of the pYeDP60 yeast expression system with a modified version of the improved uracil-excision (USER) cloning technique provides a new powerful tool for high-throughput expression of eukaryotic cytochrome P450s. The vector presented is designed to obtain an optimal 5' untranslated sequence region for yeast (Kozak consensus sequence), and has been tested to produce active P450s and NADPH-cytochrome P450 oxidoreductase (CPR) after 5' end silent codon optimization of the cDNA sequences… CONTINUE READING

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