Improved axenization method reveals complexity of symbiotic associations between bacteria and acanthamoebae.

@article{Lagkouvardos2014ImprovedAM,
  title={Improved axenization method reveals complexity of symbiotic associations between bacteria and acanthamoebae.},
  author={Ilias Lagkouvardos and Jie Shen and Matthias Horn},
  journal={Environmental microbiology reports},
  year={2014},
  volume={6 4},
  pages={
          383-8
        }
}
Bacteria associated with free-living amoebae have attracted considerable attention because of their role in human disease and as models for studying endosymbiosis. However, the identification and analysis of such novel associations are hindered by the limitations of methods for isolation and axenization of amoebae. Here, we replaced the heat-inactivated Escherichia coli, which is typically used as food source during axenization, with a live E. coli tolC knockout mutant strain hypersensitive to… CONTINUE READING

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