Improved and automated prediction of effective siRNA.

  title={Improved and automated prediction of effective siRNA.},
  author={Alistair M. Chalk and Claes Wahlestedt and Erik L. L. Sonnhammer},
  journal={Biochemical and biophysical research communications},
  volume={319 1},
Short interfering RNAs are used in functional genomics studies to knockdown a single gene in a reversible manner. The results of siRNA experiments are highly dependent on the choice of siRNA sequence. In order to evaluate siRNA design rules, we collected a database of 398 siRNAs of known efficacy from 92 genes. We used this database to evaluate previously proposed rules from smaller datasets, and to find a new set of rules that are optimal for the entire database. We also trained a regression… CONTINUE READING