Improved adapter-ligation-mediated allele-specific amplification for multiplex genotyping by using software.

Abstract

Adapter-ligation-mediated allele-specific amplification (ALM-ASA) is a potential method for multiplex SNPs typing at an ultra low cost. Here, we describe a kind of software, which designs allele-specific primers for ALM-ASA assay on multiplex SNPs. DNA sequences containing SNPs of interest are submitted into the software which contains various endonucleases… (More)
DOI: 10.1002/elps.200700619

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