Improved activity and pH stability of E. coli ATCC 11105 penicillin acylase by error-prone PCR


Penicillin G acylase is the key enzyme used in the industrial production of β-lactam antibiotics. This enzyme hydrolyzes penicillin G and related β-lactam antibiotics releasing 6-aminopenicillanic acid, which is an intermediate in the production of semisynthetic penicillins. To improve the enzymatic activity of Escherichia coli penicillin acylase… (More)
DOI: 10.1007/s00253-013-5476-7


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