Improved accuracy of the transcriptional profiling method of age grading in Aedes aegypti mosquitoes under laboratory and semi-field cage conditions and in the presence of Wolbachia infection.

@article{Caragata2011ImprovedAO,
  title={Improved accuracy of the transcriptional profiling method of age grading in Aedes aegypti mosquitoes under laboratory and semi-field cage conditions and in the presence of Wolbachia infection.},
  author={Eric Pearce Caragata and Anne Poinsignon and Luciano Andrade Moreira and Petrina H. Johnson and Yi San Leong and Scott A. Ritchie and Scott L O'Neill and Elizabeth A McGraw},
  journal={Insect molecular biology},
  year={2011},
  volume={20 2},
  pages={215-24}
}
Transcriptional profiling is an effective method of predicting age in the mosquito Aedes aegypti in the laboratory, however, its effectiveness is limited to younger mosquitoes. To address this we used a microarray to identify new gene candidates that show significant expression changes in older mosquitoes. These genes were then used to create a revised model, which upon evaluation in both laboratory and semi-field conditions, proved to have improved accuracy overall and for older mosquitoes. In… CONTINUE READING

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