Improved accumulation of poly-3- hydroxybutyrate by a recombinant cyanobacterium

@article{Takahashi2004ImprovedAO,
  title={Improved accumulation of poly-3- hydroxybutyrate by a recombinant cyanobacterium},
  author={Hideyuki Takahashi and Masato Miyake and Yutaka Tokiwa and Yasuo Asada},
  journal={Biotechnology Letters},
  year={2004},
  volume={20},
  pages={183-186}
}
Synechococcus sp. PCC7942 was transformed with genes encoding poly-3-hydroxybutyrate(PHB)-synthesizing enzymes from Alcaligenes eutrophus by using a new plasmid vector. Under photoautotrophic and nitrogen-starved conditions, PHB content of the transformant was improved by CO2-enrichment in sparging. Supplement of acetate under nitrogen-starvation enhanced its PHB content by more than 25 %. The mol wt and distribution of PHB from the cyanobacterial transformant were similar to those by… CONTINUE READING