Improved RNA preservation for immunolabeling and laser microdissection.

  title={Improved RNA preservation for immunolabeling and laser microdissection.},
  author={Amanda L. Brown and Doug William Smith},
  volume={15 12},
Microdissection techniques have the potential to allow for transcriptome analyses in specific populations of cells that are isolated from heterogeneous tissues such as the nervous system and certain cancers. Problematically, RNA is not stable under the labeling conditions usually needed to identify the cells of interest for microdissection. We have developed an immunolabeling method that utilizes a high salt buffer to stabilize RNA during prolonged antibody incubations. We first assessed RNA… CONTINUE READING