Improved Quantification of DNA Methylation Using Methylation-Sensitive Restriction Enzymes and Real-Time PCR

@article{Hashimoto2007ImprovedQO,
  title={Improved Quantification of DNA Methylation Using Methylation-Sensitive Restriction Enzymes and Real-Time PCR},
  author={Ko Hashimoto and Shoichi Kokubun and Eiji Itoi and Helmtrud I. Roach},
  journal={Epigenetics},
  year={2007},
  volume={2},
  pages={86 - 91}
}
Heterogeneity of cells with respect to the DNA methylation status at a specific CpG site is a problem when assessing methylation status. We have developed a simple two-step method for the quantification of the percent of cells that display methylation at a specific CpG site in the promoter of a specific gene. The first step is overnight digestion of genomic DNA (optimal conc. 20ng/5μl) with a relevant methylation-sensitive restriction enzyme (optimal 2 units). This is followed by real time PCR… 
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