Improved Precision of iTRAQ and TMT Quantification by an Axial Extraction Field in an Orbitrap HCD Cell

@inproceedings{Pichler2011ImprovedPO,
  title={Improved Precision of iTRAQ and TMT Quantification by an Axial Extraction Field in an Orbitrap HCD Cell},
  author={Peter Pichler and Thomas K{\"o}cher and Johann Holzmann and Thomas Moehring and Gustav Ammerer and Karl Mechtler},
  booktitle={Analytical chemistry},
  year={2011}
}
Improving analytical precision is a major goal in quantitative differential proteomics as high precision ensures low numbers of outliers, a source of false positives with regard to quantification. In addition, higher precision increases statistical power, i.e., the probability to detect significant differences. With chemical labeling using isobaric tags for relative and absolute quantitation (iTRAQ) or tandem mass tag (TMT) reagents, quantification is based on the extraction of reporter ions… CONTINUE READING

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References

Publications referenced by this paper.
Showing 1-10 of 17 references

Anal. Chem

  • P Pichler, T Kocher, +4 authors K Mechtler
  • Anal. Chem
  • 2010

Mol. Cell. Proteomics

  • Y Zhang, M Askenazi, J Jiang, C J Luckey, J D Griffin, J A Marto
  • Mol. Cell. Proteomics
  • 2010

J. Proteome Res

  • S Y Ow, M Salim, J Noirel, C Evans, I Rehman, P C Wright
  • J. Proteome Res
  • 2009

Nat. Rev. Genet

  • M Gstaiger, R Aebersold
  • Nat. Rev. Genet
  • 2009

Proteomics

  • M A Kuzyk, L B Ohlund, +5 authors C Borchers
  • Proteomics
  • 2009

Curr. Opin. Chem. Biol

  • X Han, A Aslanian, J R Yates
  • Curr. Opin. Chem. Biol
  • 2008

Mol. Cell. Proteomics J. Anal. Chem

  • M Bantscheff, M Boesche, +8 authors J Coon
  • Mol. Cell. Proteomics J. Anal. Chem
  • 2008

Proc. Natl. Acad. Sci. U.S.A

  • M Mann, N L Kelleher
  • Proc. Natl. Acad. Sci. U.S.A
  • 2008

Nat. Protoc

  • S E Ong, M Mann
  • Nat. Protoc
  • 2006

Nat. Chem. Biol

  • S E Ong, M Mann
  • Nat. Chem. Biol
  • 2005

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