Improved Photobiological H2 Production in Engineered Green Algal Cells*

@article{Kruse2005ImprovedPH,
  title={Improved Photobiological H2 Production in Engineered Green Algal Cells*},
  author={Olaf Kruse and Jens Rupprecht and Klaus P. Bader and Skye R. Thomas-Hall and Peer M. Schenk and Giovanni Finazzi and Ben Hankamer},
  journal={Journal of Biological Chemistry},
  year={2005},
  volume={280},
  pages={34170 - 34177}
}
Oxygenic photosynthetic organisms use solar energy to split water (H2O) into protons (H+), electrons (e-), and oxygen. A select group of photosynthetic microorganisms, including the green alga Chlamydomonas reinhardtii, has evolved the additional ability to redirect the derived H+ and e- to drive hydrogen (H2) production via the chloroplast hydrogenases HydA1 and A2 (H2 ase). This process occurs under anaerobic conditions and provides a biological basis for solar-driven H2 production. However… 

Figures from this paper

Synthetic rewiring of Chlamydomonas reinhardtii to improve biological H2 production
TLDR
The knockdown mutants were found to be sensitive to RES due to a perturbed RES homeostasis but interestingly showed a prolonged PSII activity (Fv/Fm) under sulfur depletion.
Chlamydomonas: Hydrogenase and Hydrogen Production
TLDR
Light-driven H2 production has the highest theoretical photon conversion efficiency and is thus of considerable biotechnological interest, however, the calculated theoretical efficiencies are still not achievable in practice, despite the implementation of a wide range of engineering strategies.
Implementation of photobiological H2 production: the O2 sensitivity of hydrogenases
  • M. Ghirardi
  • Biology, Engineering
    Photosynthesis Research
  • 2015
TLDR
The author concludes with a description of current approaches from various laboratories to incorporate multiple genetic traits into either algae or cyanobacteria to jointly address limiting factors other than the hydrogenase O2 sensitivity and achieve more sustained H2 photoproduction activity.
Hydrogenases, Nitrogenases, Anoxia, and H 2 Production in Water-Oxidizing Phototrophs
TLDR
Recent biological H2 production research efforts are rapidly elucidating the metabolic pathways that supply reductant to H2-producing enzymes, the metabolic and mechanistic requirements for maturation of the metallo-enzyme centers required in H2, and the genetic techniques required for manipulating metabolism in H1-producing organisms.
Photosynthetic Water‐Splitting for Hydrogen Production
This chapter emphasizes photobiological, H2 producing organisms and processes that are able to link photosynthetic water oxidation (reductant-generation) directly to [FeFe]-hydrogenase-catalyzed H2
High rates of photobiological H2 production by a cyanobacterium under aerobic conditions.
Among the emerging renewable and green energy sources, biohydrogen stands out as an appealing choice. Hydrogen can be produced by certain groups of microorganisms that possess functional nitrogenase
Photosynthetic H2 metabolism in Chlamydomonas reinhardtii (unicellular green algae)
  • A. Melis
  • Biology, Environmental Science
    Planta
  • 2007
TLDR
Recent advances on green algal hydrogen metabolism are summarized and approaches discussed are beginning to address the biochemistry of anaerobic H2 photoproduction, its genes, proteins, regulation, and communication with other metabolic pathways in microalgae.
Analytical approaches to photobiological hydrogen production in unicellular green algae
TLDR
The principles of photobiological hydrogen production in microalgae are provided and methods by which the interaction of photosynthesis, respiration, cellular metabolism, and H2 production in Chlamydomonas can be monitored and regulated are discussed.
Hydrogen production by Chlamydomonas reinhardtii revisited: Rubisco as a biotechnological target
TLDR
The photosynthetic enzyme ribulose 1,5-bisphosphate carboxylase/oxygenase (Rubisco) appears as suitable objective for biotechnological optimization of hydrogen production because of its relevance controlling the hydrogenase main competitor electron sink (the Calvin-Benson cycle), as well as starch accumulation and photorespiratory oxygen consumption.
...
...

References

SHOWING 1-10 OF 81 REFERENCES
Sustained photobiological hydrogen gas production upon reversible inactivation of oxygen evolution in the green alga Chlamydomonas reinhardtii.
TLDR
The work describes a novel approach for sustained photobiological production of H(2) gas via the reversible hydrogenase pathway in the green alga Chlamydomonas reinhardtii, and suggests that photoreduction of ferredoxin is followed by electron donation to theversible hydrogenase.
Biochemical and morphological characterization of sulfur-deprived and H2-producing Chlamydomonas reinhardtii (green alga)
TLDR
It is suggested that, under S-deprivation conditions, electrons derived from a residual PSII H2O-oxidation activity feed into the hydrogenase pathway, thereby contributing to the H2-production process in Chlamydomonas reinhardtii.
Effects of extracellular pH on the metabolic pathways in sulfur-deprived, H2-producing Chlamydomonas reinhardtii cultures.
TLDR
Carbon balances demonstrate that alternative metabolic pathways predominate at different pHs, and these depend on whether residual photosynthetic activity is present or not.
Sustained Photoevolution of Molecular Hydrogen in a Mutant of Synechocystis sp. Strain PCC 6803 Deficient in the Type I NADPH-Dehydrogenase Complex
TLDR
It was shown that two pathways of electron supply for H2 production operate in M55, namely photolysis of water at the level of photosystem II and carbohydrate-mediated reduction of the plastoquinone pool.
Expression of two [Fe]-hydrogenases in Chlamydomonas reinhardtii under anaerobic conditions.
TLDR
This work represents the first systematic study of expression of two algal [Fe]-hydrogenases in the same organism and shows that its catalytic site models well to the known structure of Clostridium pasteurianum CpI, including the H2-gas channel.
Hydrogen Photoproduction Is Attenuated by Disruption of an Isoamylase Gene in Chlamydomonas reinhardtii
TLDR
The results indicate that STA7 and starch metabolism play an important role in C. reinhardtii H2 photoproduction and that mere anaerobiosis is not sufficient to maintain hydrogenase gene expression without the underlying physiology, an important aspect of which is starch metabolism.
A Novel Type of Iron Hydrogenase in the Green AlgaScenedesmus obliquus Is Linked to the Photosynthetic Electron Transport Chain*
TLDR
The biochemical and genetical characterization of a new type of iron hydrogenase (HydA) in this photosynthetic organism is reported and the lack of additional Fe-S clusters in the N-terminal domain indicates a novel pathway of electron transfer.
...
...